蛋白质复杂体生产与表征的先进技术：第二卷

English | 2024 | ISBN: 3031521927 | 223 Pages | PDF EPUB (True) | 50 MB

This edited volume discusses the identification, discovery, characterization, structure determination and modeling of multicomponent macromolecular complexes, and as such, it fully complements the first volume (ISBN 978-3-319-27214-6), which targeted methods of recombinant production of protein complexes. This book is divided in 8 sections offering a selection of technologies widely used in the characterization of protein and protein-nucleic acid complexes for different purposes and at different scales. From native electrophoresis methods, that are accessible to any reasonably well-equipped laboratory, to the sophisticated setup required for structure determination by cryo-electron microscopy or X-ray crystallography, this book contains a wide variety of clearly explained analytic and preparative approaches, connected with the production techniques developed on the previous volume.

这本书讨论了多组分大分子复合物的识别、发现、表征、结构确定和建模，因此它完全补充了第一卷（ISBN 978-3-319-27214-6），该卷针对重组生产蛋白质复合物的方法进行了介绍。本书分为8个部分，提供了广泛应用于蛋白质及蛋白质-核酸复合物表征的不同目的和技术，涵盖了从易于进入的原生电泳方法到用于通过冷冻电子显微镜或X射线晶体学确定结构所需复杂设置的各种技术。这本书包含了一系列清晰解释的分析和纯化方法，与上一卷开发出来的生产技术联系在一起。

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